At about 7 days seven in utero, the sexual intercourse-related gene on the Y chromosome initiates the event in the testicles in male infants. The testicles create testosterone.

When managing for the results of perception in getting been given testosterone, Women of all ages that have received testosterone make fairer presents than Women of all ages that have not received testosterone.[one hundred thirty]

男性的第二性征包括雄激素性毛发、声音变粗、喉结出现、肩膀变宽、肌肉增加和阴茎生长。

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放射免疫测定法：睾酮主要产生于睾丸小叶曲精管之间的间质细胞，此外肾上腺皮质也能合成。当标本中加入H睾酮和睾酮抗体即产生竞争抑制作用，在反应系统中，当H睾酮和其抗体量保持恒定，则标记抗体复合物的形成量受未标记抗原量所制约，用葡聚糖加膜活性炭进行分离，使结合标记抗原与游离标记抗原分开，测定沉淀的结合部分，进行液闪仪计数（dpm）与标准相比，求出未知样品的含量。

由于睾丸间质细胞分泌雄激素，如果睾丸中没有局部高水平的雄激素，生精小管就会退化，导致不孕。因此，许多经皮雄激素贴片被应用于阴囊。

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在发育中的睾丸里的性索中，来自中胚层的上皮细胞成为塞尔托利氏细胞，辅助精子的形成。

過度超量或是長期的使用睪酮，會導致睪丸及男性生殖器萎縮，且導致永久性的陽痿。如果為女性，則會出現乳房萎縮、刺激毛髮過度生長、陰蒂肥大貌似小陰莖和长胡子等種種男性化現象。

Completion of bone maturation and termination of Answers Here progress. This happens indirectly via estradiol metabolites and consequently much more steadily in men than Women of all ages.

睪酮芳構化成雌二醇會對腦及骨這兩個人體重要的組織構成主要的影響。在骨中，雌二醇加速了軟骨成長為骨的過程，使骨端接合及完成生長的程序。在中樞神經中，雌二醇是給予下視丘的主要迴饋訊號，尤其在影響排卵素的分泌。在很多哺乳動物的腦部中，某些性別差異區域在出生前或產期的雄性化，都是受著睪酮所產生的雌二醇影響，決定將來的雄性性行為。

Much like other steroid hormones, testosterone exists in serum in two kinds. Most testosterone from the serum is sure to sexual intercourse hormone-binding globulin and also to albumin, while the remaining amount of money (about 1 percent) is free of charge, or unbound. Free testosterone is in equilibrium with certain testosterone to ensure that when no cost testosterone enters cells, some bound testosterone is immediately freed.

Misuse of those medications may cause quite a few uncomfortable symptoms and cause long-phrase dangerous health problems, like blood clots, stroke, and possibly improve the risk of prostate cancer.

Synthetic testosterone is the main drug of masculinizing hormone therapy, that is a gender-affirming cure that somebody AFAB works by using to create the secondary sex properties connected to currently being assigned male at beginning (AMAB).